| 277 | 0 | 58 |
| 下载次数 | 被引频次 | 阅读次数 |
采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Plus-Orbitrap-MS)技术快速鉴定木贼的化学成分。色谱采用赛默飞公司Hypersil GOLD C18(100 mm×2.1 mm, 1.9μm)型色谱柱，0.1%甲酸-水溶液为流动相A,乙腈为流动相B,设置流速、柱温和进样量分别为0.3 mL/min、40℃和1μL;质谱采用电喷雾离子源(ESI),正、负离子模式扫描。将化合物的保留时间、准分子离子和特征碎片离子与标准品或文献报道的化合物进行对比，共鉴定化合物70个，包括22个有机酸类、15个氨基酸类、12个生物碱类、7个核苷类、4个糖类、3个黄酮类、2个芳香醛及5个其他成分。UPLC-Q-Exactive-Orbitrap-MS技术具有快速、准确、灵敏的优势，采用该方法可快速分析鉴定木贼的化学成分，为进一步研究其药效物质基础、作用机制及产品开发提供参考依据。
Abstract：UPLC-Q-Exactive-Plus-Orbitrap-MS was used to rapidly identify the chemical constituents in Equisetum hyemale.A Hypersil GOLD C18 column(100 mm×2.1 mm, 1.9 μm) was used for gradient elution using acetonitrile(B)-0.1% formic acid solution(A) as mobile phase.The flow rate of 0.3 mL/min, column temperature was 40 ℃,and sample size was 1 μL.The electrospray ionization(ESI) source was adopted for the analysis in positive/negative ion mode.The retention time, quasi-molecular ions, and MS/MS characteristic fragment ions of the chemical constituents of Equisetum hyemale were compared with those of standards or or previously reported compounds.A total of 70 compounds were identified from Equisetum hyemale,including 22 organic acids, 15 amino acids, 12 alkaloids, 7 nucleosides, 4 carbohydrates, 3 flavonoids, 2 aromatic aldehydes and 5 other constituents.UPLC-Q-Exactive-Plus-Orbitrap-MS method is rapid, accurate and sensitive, and quickly elucidates the chemical compositions of Equisetum hyemale,which provide reference for further pharmacodynamic material basis, mechanisms of action and product development study of the crude medicine.
[1]Editorial Committee of Flora of China CAS.Flora Reipublicae Popularis Sinicae.Beijing:Science Press,2004:238.中国科学院中国植物志编辑委员会.中国植物志.北京：科学出版社，2004:238.
[2]Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia.1th Volume.Beijing:China Medical Technology Press,2020:64.国家药典委员会.中国药典(第一部).北京：中国医药科技出版社，2020:64.
[3]Pan X,Zhang C H.J.Jilin Med.Univ.,2018,39(3):2 016-2 018.潘旭，张昌浩.吉林医药学院学报，2018,39(3):2 016-2 018.
[4]Yu Y J,Zhou K,Liu D S,Zhu X J,Guo Z H,Yuan S,Shi J.Chem.Reagents,2024,46(10):63-71.于亚娟，周轲，刘东升，朱旭江，郭朝晖，袁松，石晶.化学试剂，2024,46(10):63-71.
[5]Zhang J,Wang T,Men Z Y,Mao H J,Wu Y F.China Environ.Sci.,2020,40(2):851-856.张静，王婷，门正宇，毛洪钧，吴宇峰.中国环境科学，2020,40(2):851-856.
[6]Zhang Y S,Zeng P,Cheng J R,Liu X M,Qiu Y Q,Zhang H N,Zhang Y H.J.Chin.Inst.Food Sci.Technol.,2018,18(6):272-282.张友胜，曾萍，程镜蓉，刘学铭，丘银清，张惠娜，张业辉.中国食品学报，2018,18(6):272-282.
[7]Chen J,Wu H,Liu R H,Zhang C N,Sun Y K.J.Chin.Med.Mater.,2022,45(3):639-646.陈剑，吴昊，刘润花，张晨宁，孙毅坤.中药材，2022,45(3):639-646.
[8]Zhu G H,Pang W,Chen X F,Hu J N.Cent.South Pharm.,2020,18(8):1 344-1 347.朱冠华，庞韦，程学芳，胡娟妮.中南药学，2020,18(8):1 344-1 347.
[9]Song K X.Nanopriming with Green Synthesis of Silver Effects on the Germination and Physiological Characteristics of Alfalfa(Medicago Sativa L.).Xianyang:Northwest Agriculture & Forestry University,2022.宋克晓.绿色合成纳米银引发对紫花苜蓿种子萌发及幼苗生理特性影响研究.咸阳：西北农林科技大学，2022.
[10]Hu H W,Zhao Y Y,Yang T L,Zheng Z X,Peng T,Deng F.Chin.J.Exp.Tradit.Med.Form.,2020,26(7):148-155.胡瀚文，赵永艳，杨天龙，郑振兴，彭腾，邓放.中国实验方剂学杂志，2020,26(7):148-155.
[11]Xu X M.Study on Quality Evalution of Different Species of Tibetan Medicine Berberidis Cortex Based on UPLC-Q-Exactive Orbitrap MS Metabolomics.Chengdu:Chengdu University of Traditional Chinese Medicine,2021.徐鑫梅.基于UPLC-Q-Exactive Orbitrap MS代谢物组学的多基原藏药小檗皮质量评价研究.成都：成都中医药大学，2021.
[12]Hao Z C.Screening and Application of Microorganisms that Inhibit Cyanobacteria Blooms.Tianjin:Tianjin University of Science and Technology,2021.郝忠超.抑制蓝藻水华微生物的筛选与应用.天津：天津科技大学，2021.
[13]Liu N,Tang R Y,She C,Tao T,Min Y H,Li H.Food Ferment.Ind.,2024,50(6):254-259.刘娜，唐睿艺，佘城，陶滔，闵宇航，李航.食品与发酵工业，2024,50(6):254-259.
[14]Liu M S,Yang B P,Yang W L,Cai Y,Wang K H,Peng M D,Chen B Z,Zheng G D.Chin.J.Hosp.Pharm.,2021,41(23):2 395-2 400;2 427.刘梦诗，杨得坡，杨婉玲，蔡轶，王康慧，彭梦蝶，陈柏忠，郑国栋.中国医院药学杂志，2021,41(23):2 395-2 400;2 427.
[15]Zhang J Y.Extraction Technology and Application of Gastrodin and Gastrodia Elata Polysaccharide.Lanzhou:Northwest Normal University,2022.张家义.天麻素和天麻多糖的提取工艺及应用.兰州：西北师范大学，2022.
[16]Feng F.The Molecular Mechanism of Xanthomonas Type Ⅲ Effector AvrAC to Regulate Plant Innate Immunity.Beijing:Tsinghua University,2012.冯锋.黄单胞菌效应蛋白AvrAC调节植物先天免疫的分子机制.北京：清华大学，2012.
[17]Gao H,Xie Y C,Li X Y,Shi G S.Chin.J.Pharm.Anal.,2015,35(9):1 653-1 659.高辉，谢元超，李晓燕，史国生.药物分析杂志，2015,35(9):1 653-1 659.
[18]Yan Y H,Wu T,Chen J,Abdula R,Huang L,Aisa H A.Chin.J.Exp.Tradit.Med.Form.,2021,27(21):156-166.严雅慧，吴涛，陈菊，热依木古丽·阿布都拉，黄磊，阿吉艾克拜尔·艾萨.中国实验方剂学杂志，2021,27(21):156-166.
[19]Li K L,Xiong P,Gong K Y,Peng J,Shi S L,Cai W.Nat.Prod.Res.Dev.,2020,32(2):250-256.李凯琳，熊佩，龚开妍，彭婕，史思林，蔡伟.天然产物研究与开发，2020,32(2):250-256.
[20]Vallverdú-queralt A,Verbaere A,Meudec E,Cheynier V,Sommerer N.J.Agric.Food.Chem.,2015,63(1):142-149.
[21]Chen X H,Jin M C.Chin.J.Health Lab.Technol.,2009,19(10):2 213-2 215;2 269.陈晓红，金米聪.中国卫生检验杂志，2009,19(10):2 213-2 215;2 269.
[22]Cui W H,Deng X L,Wang S Q.Chem.Res.,2023,34(4):313-318.崔维恒，邓晓兰，王思琴.化学研究，2023,34(4):313-318.
[23]Cui W H,Deng X L,Chen C Y.Henan Sci.,2023,41(7):978-984.崔维恒，邓晓兰，陈驰宇.河南科学，2023,41(7):978-984.
[24]Yang M Y.Studies on the Influence of Non-aqueous Medium on the Catalytic Properties of P.fluorescens/A.oryzae Whole-cells and Their Cytotoxicities on the Microbial Cells.Guangzhou:South China University of Technology,2014.杨美艳.非水介质对P.fluorescens/A.oryzae全细胞催化特性与细胞毒性影响的研究.广州：华南理工大学，2014.
[25]Chen J.Screening and Risk Prediction of Metabolic Markers of Thyroid Cancer and Laryngeal Cancer.Hefei:University of Science and Technology of China,2020.陈建.甲状腺癌和喉癌代谢标志物的筛选及其风险预测.合肥：中国科学技术大学，2020.
[26]Zhou W Y,Hu B C,Zhao L R,Liu Z L.J.Chin.Mass Spectrom.Soc.,2009,30(1):31-35.周维友，胡炳成，赵乐荣，刘祖亮.质谱学报，2009,30(1):31-35.
[27]Zhang Y,Deng Q,Wei M,Zhang X.Chin.J.Exp.Tradit.Med.Form.,2021,27(15):91-99.张烨，邓琦，魏敏，张旭.中国实验方剂学杂志，2021,27(15):91-99.
[28]Yang H T.Studies on Characteristic and Analysis of Chemical Components of Aster tataricus and Inhibitory Effect of Friedelan-3beta-ol on Cytochrome P450s in Human Liver Microsome.Shijiazhuang:Hebei Medical University,2016.杨浩天.紫菀化学成分表征与分析及表木栓醇对人肝微粒体CYP450酶抑制作用研究.石家庄：河北医科大学，2016.
[29]Han Z Y,Hu E M,Deng X K,Li Y,Zhang L S,Cao F,Chen J Y,Zhou F J.Sci.Technol.Food Ind.,2021,42(17):16-23.韩忠耀，胡恩明，邓先扩，李燕，张林甦，曹芳，陈建宇，周福军.食品工业科技，2021,42(17):16-23.
[30]Yang L.Qualitative and Quantitative Study on Effective Compounds of Fangji Huangqi Tang Against Adriamycin-induced Nephropathy Based on the Process in Vivo.Taiyuan:Shanxi University,2020.杨柳.基于体内过程的防己黄芪汤干预阿霉素肾病药效成分定性和定量研究.太原：山西大学，2020.
[31]Huang H Y,Kang J L,Yu Y H,Zhao P,Feng S X,Du Y,Li J S.J.Instrum.Anal.,2019,38(1):1-13.黄海英，康俊丽，余亚辉，赵鹏，冯素香，杜燕，李建生.分析测试学报，2019,38(1):1-13.
[32]Li D Q.J.Anhui Agric.Univ.,2021,48(2):333-338.李东芹.安徽农业大学学报，2021,48(2):333-338.
[33]Rao Y,Huang W P,Feng Y L,Ouyang H,Yang S L.Chin.Tradit.Pat.Med.,2019,41(6):1 455-1 462.饶颖，黄文平，冯育林，欧阳辉，杨世林.中成药，2019,41(6):1 455-1 462.
[34]Liao Q J,Wang X Q,An M Z,Li Y H,Qiao Z W,Zhou H L,Song T F.Liquor Mak.Sci.Technol.,2020,(12):65-70.廖勤俭，王小琴，安明哲，李杨华，乔宗伟，周韩玲，宋廷富.酿酒科技，2020,(12):65-70.
基本信息:
DOI：10.13822/j.cnki.hxsj.2024.0431
中图分类号:O657.63;R284.1
引用信息:
[1]张宝,李悦,蒋礼等.基于UPLC-Q-Exactive-Plus-Orbitrap-MS技术的木贼化学成分分析[J].化学试剂,2025,47(04):94-104.DOI:10.13822/j.cnki.hxsj.2024.0431.
基金信息:
贵阳市妇幼保健院优秀医学C类人才培养计划项目(筑妇医[2022]353号)