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为获取最佳的中药钩藤总黄酮提取条件,以提取量为响应值,在单因素实验研究的基础上,运用中心组合-响应面法考察了液固比、回流时间、乙醇浓度、提取温度及其交互作用对提取量的影响。研究结果表明,将液固比为23∶1的钩藤置于60%乙醇中并于80℃条件下提取2.0 h,总黄酮提取量最高,达1.281 6 mg/g。经验证,运用响应面分析法从钩藤中优选总黄酮工艺稳定且合理可行。
Abstract：In order to obtain the best extraction conditions for total flavonoids in traditional chinese medicine Uncaria rhynchophylla.Taking the extraction quantity as the response value and basing on single factor experiment,this work investigates the liquidsolid ratio,reflux time,ethanol concentration,extraction temperature and their interaction on the effect of extraction amount by CCD-RSM.The results show that the optimum conditions for the extraction of total flavonoids from Uncaria rhynchophylla are as follow:the liquid-solid ratio 23 ∶ 1,60% ethanol at 80 ℃ extracting for 2 hours,extraction amount of total flavonoids reached1.281 6 mg/g.The verification results show that the optimization process of total flavonoids from Uncaria is stable,reasonable and feasible by using the response surface analysis method.
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基本信息:
DOI：10.13822/j.cnki.hxsj.2017.04.019
中图分类号:R284.2
引用信息:
[1]杨伟群,许晓芸.中心组合-响应面法优化中药钩藤总黄酮的提取条件[J].化学试剂,2017,39(04):416-420.DOI:10.13822/j.cnki.hxsj.2017.04.019.
2017-04-15
2017-04-15