化学试剂

研究枇杷核石油醚、氯仿、乙酸乙酯、正丁醇、水等不同极性溶剂萃取物中总黄酮、总多酚的含量及其抗氧化活性相关性。采用比色法测定5种萃取物中总黄酮和总多酚含量,通过测定DPPH自由基清除率、ABTS⁺自由基清除率、OH~-自由基清除率及还原能力评价不同极性萃取物抗氧化活性的差异。结果表明,枇杷核各萃取物均有抗氧化活性,且均呈量效关系,其中乙酸乙酯萃取物清除能力最强,石油醚萃取物清除能力最弱;总多酚含量与DPPH、ABTS⁺呈显著性相关与还原能力呈极显著相关,可为枇杷核作为天然抗氧化剂的开发提供理论依据。

The correlation of the contents of total flavonoids,total polyphenols and their antioxidant activities in petroleum ether extract,chloroform extract,ethyl acetate extract,n-butanol extract and water extract from Loquat nuclear was studied.The contents of total flavonoids and total polyphenols in 5 extracts were determined by colorimetric method,and the antioxidant activities of extracts were evaluated and analyzed by the determination of DPPH radical scavenging ability,ABTS⁺ radical scavenging ability,OH~- radical scavenging ability and reducing power.All extracts from Loquat nuclear had antioxidant activity and showed a dose effect relationship.The total polyphenol content was significantly correlated with DPPH,ABTS⁺ and extremely significant correlation with reducing ability,which could provide a theoretical basis for the development of Loquat nuclear as a natural antioxidant.