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以N-氨乙基-Y-氨丙基三甲氧基硅烷和荧光素钠为原料，水热法合成了一种在450 nm处发射蓝色荧光的硅纳米粒子(DFC)。利用傅里叶变换红外光谱(FT-IR)、X-射线光电子能谱(XPS)、透射电镜(TEM)、Zeta电位、紫外-可见吸收光谱等对DFC进行结构表征和光谱性能分析。通过将DFC与铬离子(Cr3+)作用，构建了一种可以检测青霉素(Penicillin G,PG)的荧光探针Cr3+@DFC。当PG存在时，Cr3+@DFC的荧光回升。在0.1～1.0μg/mL检测浓度范围内，荧光回升效率与PG浓度呈良好的线性关系。线性方程为y=71.26x+0.478,R2=0.99,检出限为0.023μg/mL。方法操作简便、灵敏度高，可用于牛奶样品中PG的检测，具有广阔的推广及应用前景。
Abstract：The silicon nanoparticle(DFC)that emits blue fluorescence at 450 nm was synthesized by using the reagents of N-[3-(Trimethoxysilyl)propyl]ethylenediamine and fluorescein sodium salt.The structural characterization and spectral performance analysis of DFC were carried out by Fourier transform infrared spectroscopy(FT-IR),X-ray photoelectron spectroscopy(XPS),transmission electron microscope(TEM),Zeta potential and ultraviolet-visible absorption spectrum.Based on the performance DFC can be quenched by Cr3+ ion, a fluorescent probe of Cr3+@DFC for the detection of penicillin G(PG) was prepared.The fluorescence of the Cr3+@DFC probe was recovered in the presence of PG.There was a good linear relationship between fluorescence recovery efficiency and PG concentration in the range of 0.1～1.0 μg/mL.The detection limit is 0.023 μg/mL,and the linear equation was y=71.26x+0.478,R2=0.99.The method is easy to operate and has high sensitivity to detect PG in milk samples, which offers a promising application prospect.
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基本信息:
DOI：10.13822/j.cnki.hxsj.2024.0426
中图分类号:O657.3;TS252.7
引用信息:
[1]王升匀,李春荣,孟铁宏,等.基于硅纳米粒子的荧光探针高灵敏检测牛奶中的青霉素[J].化学试剂,2025,47(02):73-78.DOI:10.13822/j.cnki.hxsj.2024.0426.
基金信息:
黔南民族医学高等专科学校基金资助项目(QNYZ202144,202105,202106,202201,2022003,2022005,2023002); 贵州省中医药管理局中医药、民族医药科学技术课题资助项目(QZYY-2024-138); 贵州省科技厅基金资助项目(黔科合基础-ZK[2022]一般552)
2024-10-04
2024
2024-11-18
2024
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2024-11-25
2024-11-25
2024-11-25