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为设计合成香豆素衍生物荧光探针以实现对金属离子的高选择性、高灵敏识别，并为实际水样中Zn2+检测提供理论与应用参考，以香豆素衍生物3-(1H-苯并[d]咪唑-2-基)-7-(二乙氨基)-2H-苯并吡喃-2-酮(BDC)为荧光探针，在乙醇-水混合体系(V(乙醇)∶V(水)=1∶1)中，通过荧光光谱法研究其对多种金属离子的识别性能，同时采用Job's Plot实验与X-单晶衍射实验解析探针与目标离子的配合机制，并考察探针的线性响应范围、检出限、响应时间及实际水样适用性。研究结果表明，BDC探针对Zn2+表现出特异性识别能力，与Zn2+作用后荧光显著增强，且不受其他共存金属离子干扰;探针荧光强度与Zn2+浓度在特定范围内呈良好线性关系(R2=0.992 1)，检出限低至0.117μmol/L，响应时间短(≤0.5 min)。Job's Plot实验与X-单晶衍射实验进一步证实BDC可与Zn2+形成稳定配合物，其荧光增强效应源于配合作用引发的螯合荧光增强(CHEF)效应。综上，BDC荧光探针具备对Zn2+的高选择性、高灵敏性及快速响应特性，可成功应用于实际水样中Zn2+的检测，为香豆素类金属离子识别探针的设计与应用提供了有效借鉴。
Abstract：To design and synthesize coumarin-derived fluorescent probes with highly selectivity and sensitivity for metal ion recognition,as well as to provide theoretical and application references for Zn2+detection in real water samples,this study employed the coumarin derivative 3-( 1H-benzo[d]imidazol-2-yl)-7-( diethylamino)-2H-chromen-2-one( BDC) as a fluorescent probe.In V( ethanol) ∶ V( water) = 1 ∶ 1,the recognition performance of BDC towards various metal ions was investigated via fluorescence spectroscopy. Meanwhile,Job' s Plot analysis and X-ray single-crystal diffraction were used to analyze the complexation mechanism between the probe and the target ion. The linear response range,limit of detection( LOD),response time,and applicability of the probe in real water samples were also evaluated. The results showed that BDC exhibited specific recognition ability towards Zn2+,with a significant fluorescence enhancement upon interaction with Zn2+,with negligible interference from other coexisting metal ions. The fluorescence intensity of BDC showed a good linear relationship with Zn2+concentration within a certain range( R2= 0. 992 1),with a low LOD of 0. 117 μmol/L and a rapid response time( ≤0. 5 min).Furthermore,Job's Plot analysis and X-ray single-crystal diffraction confirmed that BDC form a stable complex with Zn2+,and the fluorescence enhancement effect was attributed to the chelation-enhanced fluorescence( CHEF) effect induced by the complexation. In conclusion,the BDC fluorescent probe possesses high selectivity,high sensitivity,and rapid response characteristics for Zn2+,and can be successfully applied to the Zn2+detection in real water samples. This study provided valuable insights for the design and application of coumarin-based fluorescent probes for metal ion recognition.
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基本信息:
DOI：10.13822/j.cnki.hxsj.2025.0220
中图分类号:X832;O657.3
引用信息:
[1]覃姣兰,梁武华,袁涛,等.一种苯并吡喃酮荧光探针对Zn~(2+)的检测研究[J].化学试剂,2025,47(12):71-76.DOI:10.13822/j.cnki.hxsj.2025.0220.
基金信息:
百色市科学研究与技术开发计划自筹经费项目(BK20232051; BK20232047); 大学生创新创业训练计划项目(国家级项目：202410609022;区级项目：202310609143)