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采用微波辅助低共熔溶剂(DES)对橘红花中的柚皮苷进行提取，在单因素试验结果的基础上，采用Box-Behnken响应面法对提取工艺进行优化，并研究柚皮苷对黄嘌呤氧化酶(XOD)的体外抑制作用。结果表明，橘红花柚皮苷得率的最佳工艺条件为：以氯化胆碱-乙醇为提取溶剂(物质的量比为1∶2),含水量38%,液料比20∶1(mL/g),微波时间20 s,微波功率为350 W。在该条件下橘红花柚皮苷得率为4.42%,与预测值接近。体外抑制XOD实验结果表明，柚皮苷对XOD的IC50为2.229μg/mL。提取工艺稳定可靠，可用于橘红花柚皮苷的提取，并且柚皮苷具有较强的抑制XOD活性，为橘红花的进一步开发利用提供了科学依据。
Abstract：Microwave-assisted deep eutectic solvents(DES) were employed to extract naringin from Citrus grandis(L.)Osbeck flowers.Based on the results of single-factor experiments, the extraction process was optimized by Box-Behnken response surface methodology, and the inhibitory of naringin against xanthine oxidase(XOD) was studied.The results showed that the optimum conditions for the extraction of naringin were as follows: choline chloride-ethanol as the extraction solvent at a molar ratio of 1∶2 with a water content of 38%,the solvent-material ratio of 20∶1(mL/g),the microwave time of 20 s, and the microwave power of 350 W.Under this condition, the extraction yield was 4.42%,which was close to the predicted value.The in vitro results of XOD inhibition demonstrated that the IC50 of naringin on OXD was 2.229 μg/mL.This extraction process is stable and reliable, which can be used for the extraction of naringin from Citrus grandis(L.)Osbeck flowers.Moreover, naringin has strong inhibitory against XOD.This study provides a scientific evidence for the further development and utilization of Citrus grandis(L.)Osbeck flowers.
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基本信息:
DOI：10.13822/j.cnki.hxsj.2023.0480
中图分类号:R284.2
引用信息:
[1]臧青民,黄雅曼,杨瑞清,等.微波辅助低共熔溶剂优化橘红花柚皮苷提取工艺及其对黄嘌呤氧化酶抑制活性研究[J].化学试剂,2023,45(12):54-61.DOI:10.13822/j.cnki.hxsj.2023.0480.
基金信息:
广西科技基地和人才专项项目(桂科AD20159090); 玉林师范学院高层次人才科研启动基金项目(G2018016)
2023-10-23
2023-10-23
2023-10-23